Clinical significance of T lymphocyte subsets, immunoglobulin and complement expression in peripheral blood of children with steroid-dependent nephrotic syndrome/frequently relapsing nephrotic syndrome.

OBJECTIVE

To investigate the clinical significance of T lymphocyte subsets, immunoglobulin and complement expression in the peripheral blood of children with steroid-dependent nephrotic syndrome/frequently relapsing nephrotic syndrome (SDNS/FRNS).

METHODS

A prospective study was conducted on 285 children with nephrotic syndrome (NS). Among the 285 patients, 187 children had steroid-sensitive nephrotic syndrome (SSNS) and 98 children had SDNS/FRNS according to their sensitivity to hormones. Meanwhile, 50 healthy children in the same period were selected as the control group. Serum albumin (ALB), blood urea nitrogen (BUN), serum creatinine (SCr), estimated glomerular filtration rate (eGFR), high-sensitivity C-reactive protein (hs-CRP), interleukin-6 (IL-6), CD3+, CD4+, CD8+, immunoglobulin IgA, IgG, IgM and complement C3 and C4 were measured upon admission, and the content of urinary CD80 was also determined.

RESULTS

Compared with the control group, BUN, SCr, hs-CRP and IL-6 levels, urinary CD80, IgA, IgM and C3 in the SDNS/FRNS and SSNS groups were significantly higher, while ALB, eGFR, CD3+, CD4+, CD4+/CD8+, IgG and IgG/IgM were significantly lower (all P<0.05). Compared with the SSNS group, BUN, SCr, hs-CRP and IL-6 levels in the SDNS/FRNS group were significantly higher, while ALB and eGFR levels were significantly lower (all P<0.05). Compared with the SDNS/FRNS group, IgM in the SSNS group was significantly lower, while CD4+/CD8+, urinary CD80 and IgG/IgM were significantly higher (all P<0.001).

CONCLUSION

Renal function decline and inflammatory response existed in children with NS. CD3+, CD4+, CD4+/CD8+ and IgG/IgM in peripheral blood were decreased, while IgA, IgM, C3 and urinary CD80 were increased. Moreover, renal function decline, increase of inflammatory factors, decrease of IgG/IgM and CD4+/CD8+ were more obvious in the SDNS/FRNS group.