Comparison of the responses to the sensory neuropeptides, substance P, neurokinin A, neurokinin B and calcitonin gene-related peptide and to trigeminal nerve stimulation in the iris sphincter muscle of the rabbit.

Three mammalian tachykinins (substance P, neurokinin A and B) and two non-mammalian ones (eledoisin and physalaemin) produced potent contractions of the isolated rabbit iris sphincter muscle. The rank order of potencies was eledoisin greater than neurokinin B = physalaemin greater than substance P greater than neurokinin A. The maximum efficacy was much the same. The contractile responses to neurokinin A and eledoisin developed more rapidly than did those to the other tachykinins used and were selectively attenuated by [D-Arg1, D-Pro2, D-Trp7,9, Leu11]-SP. Electrical transmural stimulation produced a contraction consisting of cholinergic and tachykininergic components. The tachykininergic component was abolished by pretreatment with capsaicin or by trigeminal denervation (Fujiwara et al., 1984). [D-Arg1, D-Pro2, D-Trp7,9, Leu11]-SP attenuated the tachykininergic component, but not the cholinergic one. KCl and capsaicin also produced a tachykininergic contraction which was inhibited by [D-Arg1, D-Pro2, D-Trp7,9, Leu11]-SP. Calcitonin gene-related peptide affected neither the iris sphincter muscle nor the response to electrical transmural stimulation. These results suggest that the tachykininergic responses induced by electrical transmural stimulation, KCl and capsaicin are predominantly mediated by neurokinin A, probably released from the peripheral endings of trigeminal nerves.