Plasmacytoma variant translocation 1 (PVT1) regulates trophoblast viability, proliferation, and migration and is downregulated in spontaneous abortion.

PROBLEM: Human pregnancy is a complex biological process, and spontaneous abortion is the most common complication of pregnancy. LncRNAs have been identified that play key roles in a variety of human diseases. Recently, lncRNAPVT1 was reported to relate to the pathogenesis and progression of pregnancy. However, the role and underlying mechanism of PVT1 in trophoblast cell dysfunction in spontaneous abortion remain largely unknown.

METHOD OF STUDY: The effects of PVT1, miR-424, and eIF5A on HTR8 cells and human villi tissues from spontaneous or induced abortions were investigated using CCK-8 assay, EdU assay, real-time polymerase chain reaction, Western blotting, cell transfection assays, cell migration assays, and luciferase reporter gene assays.

RESULTS

Overexpression of PVT1 promoted HTR8 cell viability, proliferation, and migration. Suppression of PVT1 promoted miR-424 expression, and miR-424 could modulate the effects of PVT1 in HTR8 cells. MiR-424 exerted its function via regulation of eIF5A expression in HTR8 cells. PVT1 and eIF5A expression were decreased and miR-424 was increased in clinical samples from spontaneous abortion, compared to samples from elective induced abortion.

CONCLUSION

PVT1 regulates trophoblast cell function via modulation of a PVT1/miR-424/eIF5A pathway.