Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases · 2007
[Preparation of five ginkgolic acid monomers and their molluscicidal effects against Oncomelania hupensis].
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Mao Zuo-hua, Yu Pei-zhong, Sun Kai, Pan Xiao-hai, Jiang Qing-wu, Pan Jia-hu
Аннотация
OBJECTIVE: To investigate the molluscicidal activities of the ginkgolic acid(GA) monomers isolated and purified from GAs.
METHODS: Five monomers of GAs from the sarcotesta of Ginkgo biloba. were extracted by petrol ether, separated by silica gel column chromatography, purified by semi-prepared reversed-phased HPLC, and identified by LC-MS analysis. The molluscicidal activities of GAs and their monomers against Oncomelania hupensis were determined as referring to the WHO guidelines for laboratory molluscicidal test.
RESULTS: The five purified ginkgolic acid monomers were GA(13:0), GA(15:0), GA(15:1), GA(17:1) and GA(17:2), with a side chain of 13, 15, 17 alkyl or ethylenic radicals res pectively on their benzene loop. The five monomer proportions to the total GAs were 17.6%, 3.2%, 52.3%, 23.3% and 3.6% respectively. The order of molluscicidal activities for the five monomers was as follows: GA(13:0)>GA(15:1)>GA(15:0)>GA(17:1)>GA(17:2), and their LC50 for snails was 20.79 mg/L, 22.28 mg/L, 33.76 mg/L, 51.89 mg/L, and 59.10 mg/L respectively after immersion for 24 hours. Two monomers, GA(13:0), and GA(15:1) inhibited the snails' climbing up significantly.
CONCLUSION: The molluscicidal activities of GAs may be dependent on the monomer's structure with different number of carbon molecules and double-bonds on the side carbon-chain. The two monomers, GA(13:0) and GA(15:1), are mainly responsible for the molluscicidal activities of GAs and both effectively inhibit snails' climbing up as well. GA(15:0) also shows certain molluscicidal activity.
METHODS: Five monomers of GAs from the sarcotesta of Ginkgo biloba. were extracted by petrol ether, separated by silica gel column chromatography, purified by semi-prepared reversed-phased HPLC, and identified by LC-MS analysis. The molluscicidal activities of GAs and their monomers against Oncomelania hupensis were determined as referring to the WHO guidelines for laboratory molluscicidal test.
RESULTS: The five purified ginkgolic acid monomers were GA(13:0), GA(15:0), GA(15:1), GA(17:1) and GA(17:2), with a side chain of 13, 15, 17 alkyl or ethylenic radicals res pectively on their benzene loop. The five monomer proportions to the total GAs were 17.6%, 3.2%, 52.3%, 23.3% and 3.6% respectively. The order of molluscicidal activities for the five monomers was as follows: GA(13:0)>GA(15:1)>GA(15:0)>GA(17:1)>GA(17:2), and their LC50 for snails was 20.79 mg/L, 22.28 mg/L, 33.76 mg/L, 51.89 mg/L, and 59.10 mg/L respectively after immersion for 24 hours. Two monomers, GA(13:0), and GA(15:1) inhibited the snails' climbing up significantly.
CONCLUSION: The molluscicidal activities of GAs may be dependent on the monomer's structure with different number of carbon molecules and double-bonds on the side carbon-chain. The two monomers, GA(13:0) and GA(15:1), are mainly responsible for the molluscicidal activities of GAs and both effectively inhibit snails' climbing up as well. GA(15:0) also shows certain molluscicidal activity.