Asian Pacific journal of cancer prevention : APJCP · 2025
Synergistic Chemopreventive Effects of Curcumin, Gingerol, and Shogaol on HeLa Cervical Cancer Cells.
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Abdull Rahim Unwaniah, Mohd Yusof Yasmin Anum, Mustapa Marami, Nurdin Armania, Che Roos Nur Aishah, Mohamad Taridi Nursiati, Mad Nordin Mariam Firdhaus, Mohamed Shakrin Nik Noorul Shakira
Аннотация
OBJECTIVE: Bioactive compounds including curcumin, gingerols, and shogaols possess strong anti-inflammatory and anti-tumour properties. However, their combined efficacy has yet to be fully explored. This study aimed to investigate the chemopreventive potential of individual and combined treatments of 6-/10-gingerols (6-/10-G), 6-/10-shogaols (6-/10-S), and curcumin (Cur) in HeLa cervical cancer cells.
METHODS: HeLa cell viability was evaluated using the MTT assay following a 24-hour treatment with a wide range of concentrations and ratios of the bioactive compounds. The combination index was analysed using the Chou-Talalay method, and apoptotic cells were assessed via flow cytometry with Annexin V/FITC-PI staining.
RESULTS: We found that the optimal formulation of Cur in combination with all ginger bioactive compounds demonstrated synergistic inhibition of HeLa cancer cell growth at a 3:1 ratio of Cur to ginger bioactive compounds. Furthermore, treatment with the Cur:6-G (45µM: 40µM) and Cur:10-S (45µM: 20µM) combination formulations at a ratio of 3:1 significantly (p < 0.005) inhibited cell growth (72-77%) and induced apoptosis (60-87%). These findings highlight the potent anticancer properties of the combined formulations on HeLa cells, surpassing the efficacy of individual compounds which require relatively high concentrations.
CONCLUSION: The combination of Cur and ginger bioactive compounds enhances anti-proliferative effects and induces apoptosis in HeLa cell lines.
METHODS: HeLa cell viability was evaluated using the MTT assay following a 24-hour treatment with a wide range of concentrations and ratios of the bioactive compounds. The combination index was analysed using the Chou-Talalay method, and apoptotic cells were assessed via flow cytometry with Annexin V/FITC-PI staining.
RESULTS: We found that the optimal formulation of Cur in combination with all ginger bioactive compounds demonstrated synergistic inhibition of HeLa cancer cell growth at a 3:1 ratio of Cur to ginger bioactive compounds. Furthermore, treatment with the Cur:6-G (45µM: 40µM) and Cur:10-S (45µM: 20µM) combination formulations at a ratio of 3:1 significantly (p < 0.005) inhibited cell growth (72-77%) and induced apoptosis (60-87%). These findings highlight the potent anticancer properties of the combined formulations on HeLa cells, surpassing the efficacy of individual compounds which require relatively high concentrations.
CONCLUSION: The combination of Cur and ginger bioactive compounds enhances anti-proliferative effects and induces apoptosis in HeLa cell lines.