Natural needle microstructure-based immunochromatographic assay for sensitively detecting streptomycin in food products.

Immunochromatographic assays (ICAs) are famous as the point of care test (POCT) technology against food fraud for antibiotics detection. However, the poor sensitivity, stability and the monoclonal antibodies (mAbs)-depended signals limited their further applications. Here, inspired by the vivid color display and functionalization of natural pigments, we developed natural edible pigment-derived needle microstructure (NNMS) as signal tracers by simple crosslinking of alizarin and formaldehyde, allowing high color rendering ability, significant water dispersibility and antibody enrichment. Particularly, immune-network technology was introduced to achieve the simultaneous promotion of colorimetric signal and sensitivity and avoid visual errors in ICA. As a result, the prepared eco-friendly and sustainable NNMS-ICA showed satisfactory performance (LOD was 0.56 ng mL-1), specificity and probe stability (CV was 1.90 %). Furthermore, the NNMS-ICA was successfully applied in food samples (milk and honey) with total recoveries at 91.00-118.82 %.