Journal of pediatric surgery · 2010
Effects of estrogen on lung development in a rat model of diaphragmatic hernia.
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Chen Gong, Qiao Yinli, Xiao Xianmin, Zheng Shan, Chen Lian
Аннотация
PURPOSE: The study aimed to observe the influence of estradiol on rat models with congenital diaphragmatic hernia (CDH) and understand the potential mechanism.
METHODS: Eleven pregnant female Sprague-Dawley rats were randomly divided into 3 groups on day 9.5 of gestation: group C (n = 2) was administered 2 mL of olive oil, whereas group N (n = 3) and group E (n = 6) were administered 200 mg of nitrofen. Antenatal estradiol was given subcutaneously to group E on days 18.5, 19.5, and 20.5 of gestation. Histologic evaluations, incidence of CDH, and the immunoreactivity of transforming growth factor (TGF)-β1 in lung were observed. In addition, the mRNA levels of TGF-β1, type I TGF-β receptor (TβRI), and type II TGF-β receptor (TβRII) were determined.
RESULTS: Histologically, the lungs of group N fetuses were hypoplastic compared with those of group C and had thick-walled septa with poorly developed saccules. Group E showed improved mesenchymal differentiation with well-developed saccules. There was no significant difference between the incidence of CDH in group N and that in group E. The expression of TGF-β1 in lung tissue and arterioles in group N were significantly higher than those in group C and E. Moreover, relative mRNA expression levels of TGF-β1 and TβRI in group N were markedly higher than those in group C, whereas those in group E were significantly decreased compared with group N.
CONCLUSIONS: Estradiol can promote lung development in rats with CDH. The down-regulation of TGF-β1 and its signaling pathway may play a role in this effect.
METHODS: Eleven pregnant female Sprague-Dawley rats were randomly divided into 3 groups on day 9.5 of gestation: group C (n = 2) was administered 2 mL of olive oil, whereas group N (n = 3) and group E (n = 6) were administered 200 mg of nitrofen. Antenatal estradiol was given subcutaneously to group E on days 18.5, 19.5, and 20.5 of gestation. Histologic evaluations, incidence of CDH, and the immunoreactivity of transforming growth factor (TGF)-β1 in lung were observed. In addition, the mRNA levels of TGF-β1, type I TGF-β receptor (TβRI), and type II TGF-β receptor (TβRII) were determined.
RESULTS: Histologically, the lungs of group N fetuses were hypoplastic compared with those of group C and had thick-walled septa with poorly developed saccules. Group E showed improved mesenchymal differentiation with well-developed saccules. There was no significant difference between the incidence of CDH in group N and that in group E. The expression of TGF-β1 in lung tissue and arterioles in group N were significantly higher than those in group C and E. Moreover, relative mRNA expression levels of TGF-β1 and TβRI in group N were markedly higher than those in group C, whereas those in group E were significantly decreased compared with group N.
CONCLUSIONS: Estradiol can promote lung development in rats with CDH. The down-regulation of TGF-β1 and its signaling pathway may play a role in this effect.
Предметные рубрики MeSH
AnimalsArteriolesDrug Evaluation, PreclinicalEstradiolFemaleFetal Organ MaturityGene Expression Regulation, DevelopmentalHernia, DiaphragmaticHernias, Diaphragmatic, CongenitalInjections, SubcutaneousLungPhenyl EthersPregnancyProtein Serine-Threonine KinasesRNA, MessengerRatsRats, Sprague-DawleyReceptor, Transforming Growth Factor-beta Type IReceptor, Transforming Growth Factor-beta Type IIReceptors, Transforming Growth Factor betaReverse Transcriptase Polymerase Chain ReactionTransforming Growth Factor beta1