Optimization of Dendrimer Polyamidoamin Electrospun Nanofibers: Preparation and Properties.

BACKGROUND

The most common polymers in the treatment of wounds are natural (e.g., polysaccharides, proteins, and peptides) and synthetic polymers (e.g., poly-glycolic acid, polyacrylic acid, polylactic acid, and polyvinyl alcohol) due to their biodegradability, biocompatibility, and their structural resemblance to the macromolecules known to the human body.

OBJECTIVES

The current study aimed to develop an electrospinning method using the nanofibers of polyvinyl alcohol (PVA)/carboxymethyl cellulose (CMC)/polyamide amine (PAMAM)/tetracycline (Tet) to cover the wound. The antibacterial effect of PAMAM was also tested against E. coli and S. aureus bacteria.

MATERIALS AND

METHODS

The morphology of the composite nanofiber was studied by a field emission scanning electron microscope. Infrared spectroscopy (FTIR) was used to characterize the nano chemical structure.

RESULTS

Nanofibers were evaluated based on the release of different amounts of the antibiotic tetracycline (1%, 3%, 5%, and 7% by weight) while preventing wound infection. The findings indicated that the highest-profile release of all nanofibers occurred early within 12 hours. It was found that nanofiber membranes loaded with 1%, 3%, and 5% tetracycline released drugs for over 28 days, while those containing 7% tetracycline released drugs for more than 14 days.

CONCLUSIONS

According to the findings related to the drug release of PVA/CMC/15% PAMAM/Tet and surface morphology of the nanofibers, the optimal amount of Tet was 5%. The results of FTIR spectroscopy indicated that the tetracycline and polyamidoamine were successfully placed in nanofibers.