The application of continuous wavelet transform based on spectrophotometric method and high-performance liquid chromatography for simultaneous determination of anti-glaucoma drugs in eye drop.

In this study, a fast, low-cost, accurate, and precise spectrophotometric method based on the continuous wavelet transform (CWT) was assayed to determine dorzolamide (DOR) and timolol (TIM) in an eye drop sample simultaneously. Different wavelet families were investigated to select the best family for analyzing the DOR and TIM. The Mexican hat wavelet (MHW) family with the wavelength of 281 nm and Gaussian wavelet family (gaus2) in the wavelength of 267 nm were found for the simultaneous analysis of DOR and TIM, respectively. Mean recovery values of synthetic mixtures were found 97.44%±2.63 and 99.18%±4.00 for DOR and TIM, respectively. The root mean square errors (RMSE) of DOR and TIM were achieved 0.5550 and 0.3306, respectively. Eye drop as a real sample was analyzed by spectrophotometry coupled with the CWT technique, as well as high-performance liquid chromatography (HPLC) as a reference method. The obtained results were compared with each other by the one-way analysis of variance (ANOVA) test and there was no significant difference between them.