Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology · 2007
[Preparation and identification of monoclonal antibodies against snake venom C-type lectin like protein Agkisacutacin].
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Su Li-Jing, Xiao Liang, Zhan Tao, Chen Lan, Dong Yan-Qiu, Fang Li, Cheng Lian-Sheng, Liu Jing
Аннотация
AIM: To prepare and identify the antibodies against snake venom C-type lectin like protein Agkisacutacin.
METHODS: A BALB/c mouse was immunized with Agkisacutacin and the cells were fused by standard hybridoma technique to prepare mAbs. The stability of the hybridomas secreting mAbs was detected by indirect ELISA. The nuclear type of the hybridomas was analyzed by fluorescent staining and the specificity of mAbs was detected by Western blot.
RESULTS: Three cell strains of hybridomas that could steadily secrete anti-Agkisacutacin mAb were obtained.
CONCLUSION: The successful preparation of anti-Agkisacutacin mAbs provides an important tool for studying the in vivo metabolism of new anti-thrombus drugs by detecting Agkisacutacin and investigating the mechanism of anti-thrombus of the drugs.
METHODS: A BALB/c mouse was immunized with Agkisacutacin and the cells were fused by standard hybridoma technique to prepare mAbs. The stability of the hybridomas secreting mAbs was detected by indirect ELISA. The nuclear type of the hybridomas was analyzed by fluorescent staining and the specificity of mAbs was detected by Western blot.
RESULTS: Three cell strains of hybridomas that could steadily secrete anti-Agkisacutacin mAb were obtained.
CONCLUSION: The successful preparation of anti-Agkisacutacin mAbs provides an important tool for studying the in vivo metabolism of new anti-thrombus drugs by detecting Agkisacutacin and investigating the mechanism of anti-thrombus of the drugs.