Methylobacterium oryzisoli sp. nov., a novel methylotrophic methanol-utilizing bacterium isolated from the paddy soil.

Four pink-pigmented bacteria, designated as strains sgz302003T, sgz302004, sgz302005 and sgz302006, were isolated from a paddy soil enrichment. The cells were aerobic, Gram-stain-negative, motile and short rods. The ranges and optima of the temperature, NaCl concentration and pH for growth were 25-37 °C (optimum, 30 °C), 0-2% (w/v) (optimum, 0-0.5%) and pH 5.0-10.0 (optimum, pH 6.5), respectively. Major fatty acids were summed feature 8 (C18:1 ω7c and/or C18:1 ω6c). Results of phylogenetic analysis based on 16S rRNA gene sequences indicated that the four strains were affiliated with the genus Methylobacterium and were most closely related to Methylobacterium isbiliense AR24T (99.2% sequence similarity), Methylobacterium nodulans ORS 206T (97.7%) and Methylobacterium currus PR1016AT (97.4%). The methanol dehydrogenase mxaF gene was absent, but xoxF was present. Phylogenomic tree supported that these four strains formed a distinct clade within Methylobacterium. Q-10 was detected as the only respiratory quinone in the studied strains. The genomic DNA G+C content was 70.7-70.8 %. The orthologous average nucleotide identity and in silico digital DNA-DNA hybridization values between the four strains and M. isbiliense AR24T were 91.8-91.9% and 53.0-53.3%, respectively, while those with M. nodulans ORS 206T were 86.3-86.5% and 25.5%. Phylogenomic, physiological and chemotaxonomic analyses indicate that the four strains represent a novel species within the genus Methylobacterium, for which the name Methylobacterium oryzisoli sp. nov. is proposed, with strain sgz302003T (= MCCC 1K09384T=KCTC 8693T) as the type strain.