Future science OA · 2017
In silico functional elucidation of uncharacterized proteins of Chlamydia abortus strain LLG.
СтатьяНазвание и аннотация: Перевод в очереди
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Singh Gagandeep, Sharma Dixit, Singh Vikram, Rani Jyoti, Marotta Francessco, Kumar Manoj, Mal Gorakh, Singh Birbal
Аннотация
AIM: This study reports structural modeling, molecular dynamics profiling of hypothetical proteins in Chlamydia abortus genome database.
METHODOLOGY: The hypothetical protein sequences were extracted from C. abortus LLG Genome Database for functional elucidation using in silico methods.
RESULTS: Fifty-one proteins with their roles in defense, binding and transporting other biomolecules were unraveled. Forty-five proteins were found to be nonhomologous to proteins present in hosts infected by C. abortus. Of these, 31 proteins were related to virulence. The structural modeling of two proteins, first, WP_006344020.1 (phosphorylase) and second, WP_006344325.1 (chlamydial protease/proteasome-like activity factor) were accomplished. The conserved active sites necessary for the catalytic function were analyzed.
CONCLUSION: The finally concluded proteins are envisioned as possible targets for developing drugs to curtail chlamydial infections, however, and should be validated by molecular biological methods.
METHODOLOGY: The hypothetical protein sequences were extracted from C. abortus LLG Genome Database for functional elucidation using in silico methods.
RESULTS: Fifty-one proteins with their roles in defense, binding and transporting other biomolecules were unraveled. Forty-five proteins were found to be nonhomologous to proteins present in hosts infected by C. abortus. Of these, 31 proteins were related to virulence. The structural modeling of two proteins, first, WP_006344020.1 (phosphorylase) and second, WP_006344325.1 (chlamydial protease/proteasome-like activity factor) were accomplished. The conserved active sites necessary for the catalytic function were analyzed.
CONCLUSION: The finally concluded proteins are envisioned as possible targets for developing drugs to curtail chlamydial infections, however, and should be validated by molecular biological methods.