Acta informatica medica : AIM : journal of the Society for Medical Informatics of Bosnia & Herzegovina : casopis Drustva za medicinsku informatiku BiH · 2022
Study of Small Non-Coding RNA (miRNA) Expression Pattern of Fertile/Infertile Male Semen.
СтатьяНазвание и аннотация: Перевод в очереди
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Al-Mawlah Yasir Haider, Al-Darraji Mustafa Nuhad, Al-Imari Mustafa Jawad
Аннотация
BACKGROUND: Infertility is a serious health issue that affects people all around the world. One of the most common reasons for male infertility is sperm abnormalities. Researchers and scientists have been searching for a novel genetic marker to detect or recognize the genetic malfunction that causes sperm abnormalities. Micro-RNA (miRNAs) are small non-coded RNA molecules that present intra and extra-cellular and regulate gene expression.
OBJECTIVE: This studies began to search for a relation between miRNA expression levels and other diseases that may be related to them, considering that the main role of miRNAs was the down-regulation of genes.
METHODS: The main technique used in this study was to synthesize a complementary DNA (cDNA) (revers transcription method) of extracted total RNA by TRIzol then amplification of candidates' miRNAs genes by Reverse Transcriptase Quantitative Polymerase Chain Reaction RT-qPCR.
RESULTS: Studies found that miRNAs have a role in defining sperm qualities such as sperm count, motility, and shape. In this study, we chose the most miRNAs referred to in the previous study as a potential seminal fluid marker (miR-10a, miR-10b, miR-135a and miR-135b) to test them as potential infertility-related miRNAs markers (Asthenospermia AS, Oligospermia OS, Astheno-Oligospermia ASOS) in addition to health normal sperm NS.
CONCLUSION: the main aim of this study was to find the miRNAs expression pattern to find a way to help scientists track the genetic causes of male infertility issues and a novel method to distinguish infertility genetically diseases.
CONCLUSION: The findings may serve as a potential genetic marker for male infertility and provide a background for future research that targeted miRNAs as a molecular marker for medical and forensic fields, also as an infertility disease potential treatment.
OBJECTIVE: This studies began to search for a relation between miRNA expression levels and other diseases that may be related to them, considering that the main role of miRNAs was the down-regulation of genes.
METHODS: The main technique used in this study was to synthesize a complementary DNA (cDNA) (revers transcription method) of extracted total RNA by TRIzol then amplification of candidates' miRNAs genes by Reverse Transcriptase Quantitative Polymerase Chain Reaction RT-qPCR.
RESULTS: Studies found that miRNAs have a role in defining sperm qualities such as sperm count, motility, and shape. In this study, we chose the most miRNAs referred to in the previous study as a potential seminal fluid marker (miR-10a, miR-10b, miR-135a and miR-135b) to test them as potential infertility-related miRNAs markers (Asthenospermia AS, Oligospermia OS, Astheno-Oligospermia ASOS) in addition to health normal sperm NS.
CONCLUSION: the main aim of this study was to find the miRNAs expression pattern to find a way to help scientists track the genetic causes of male infertility issues and a novel method to distinguish infertility genetically diseases.
CONCLUSION: The findings may serve as a potential genetic marker for male infertility and provide a background for future research that targeted miRNAs as a molecular marker for medical and forensic fields, also as an infertility disease potential treatment.