Changes in store-operated calcium channels in rat bladders with detrusor overactivity.

OBJECTIVE

To investigate the regulation of intracellular store-operated calcium channels (SOCCs) in detrusor overactivity (DO) during detrusor function changes in Sprague-Dawley rats.

METHODS

Sixty female Sprague-Dawley rats were randomized into control and DO groups. The contraction of the smooth muscle of the bladder was evaluated in vivo using smooth muscle strips. Changes in intracellular calcium ions were observed using confocal microscopy with preload fluo-4 AM, the SOCC agonist cyclopiazonic acid (CPA; 10 μM) and inhibitor SKF-96365 (10 μM). Cell currents were recorded with the whole-cell patch-clamp technique.

RESULTS

The in vitro frequencies of bladder smooth muscle contraction were significantly different (P <.05) between the DO and control groups, and the amplitudes were not significantly different (P >.05). The changes in intracellular calcium ions and current density were significantly different between the 2 groups (P <.05).

CONCLUSION

SOCCs were involved in DO and caused variations in muscle contraction.