Journal of autoimmunity · 2020
Comprehensive TCR repertoire analysis of CD4+ T-cell subsets in rheumatoid arthritis.
СтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод в очереди
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Jiang Xu, Wang Shiyu, Zhou Chen, Wu Jinghua, Jiao Yuhao, Lin Liya, Lu Xin, Yang Bo, Zhang Wei, Xiao Xinyue, Li Yueting, Wu Xunyao, Wang Xie, Chen Hua, Zhao Lidan, Fei Yunyun, Yang Huaxia, Zhang Wen, Zhang Fengchun, Chen Hui, Zhang Jianmin, Li Bin, Yang Huanming, Wang Jian, Liu Xiao, Zhang Xuan
Аннотация
The pathogenesis of rheumatoid arthritis (RA), a systemic autoimmune disease characterized by autoreactive T-cell accumulation and pro-inflammatory cytokine overproduction, is unclear. Systematically addressing T-cell receptor (TCR) repertoires of different CD4+ T-cell subsets could help understand RA pathogenesis. Here, peripheral CD4+ T cells from treatment-naïve RA patients and healthy controls were sorted into seven subsets including naïve, effector, central memory, effector memory (EMT), Th1, Th17, and regulatory T cells. T-cell receptor β chain repertoires were then analyzed by next-generation sequencing. We identified T-cell clonal expansion in EMT and Th17 cells of RA patients, with highly similar TCR repertoires. Ex vivo experiments demonstrated the preferred differentiation from EMT to Th17 cells in RA. Notably, we showed that TCR diversity and abundance of differentiated T cells of Th17 were significantly correlated with RA disease activity. Based on these observations, we propose that abnormal differentiation from EMT to Th17 and expansion of Th17 play pivotal role in RA pathogenesis.