Journal of the science of food and agriculture · 2020
Aptamer-based ellipsometric sensor for ultrasensitive determination of aminoglycoside group antibiotics from dairy products.
Оценочное исследованиеСтатьяНазвание и аннотация: Перевод в очереди
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Caglayan Mustafa Oguzhan
Аннотация
BACKGROUND: Residual antibiotics taken along with food consumed through the food chain are the main cause of the super-bacteria and may damage organs such as liver and kidney. Therefore, monitoring residual antibiotic levels of products in the food chain is both important and a requirement. Maximum residual limits for kanamycin and neomycin are 150 ng mL-1 and 500 ng mL-1 respectively, which are challenging for most sensor platforms. In this paper, a novel method is presented for the determination of antibiotics residues in animal-derived foods.
RESULTS: Aptamer-based kanamycin and neomycin biosensors based on the spectroscopic ellipsometer and the surface plasmon resonance-enhanced total internal reflection ellipsometer methods as transducing element were developed. Detection limits of both sensor platforms were in the 0.1-1 nmol L-1 ranges, and the detection range was between the detection limit and 1000 nmol L-1 .
CONCLUSION: Both ellipsometry-based aptasensors can be used as an alternative to the existing enzyme-linked immunosorbent assay-based method in terms of assay time (10 min), detection limit (0.22 ng mL-1 for neomycin and 0.048 ng mL-1 for kanamycin), and detection range. © 2020 Society of Chemical Industry.
RESULTS: Aptamer-based kanamycin and neomycin biosensors based on the spectroscopic ellipsometer and the surface plasmon resonance-enhanced total internal reflection ellipsometer methods as transducing element were developed. Detection limits of both sensor platforms were in the 0.1-1 nmol L-1 ranges, and the detection range was between the detection limit and 1000 nmol L-1 .
CONCLUSION: Both ellipsometry-based aptasensors can be used as an alternative to the existing enzyme-linked immunosorbent assay-based method in terms of assay time (10 min), detection limit (0.22 ng mL-1 for neomycin and 0.048 ng mL-1 for kanamycin), and detection range. © 2020 Society of Chemical Industry.