[Isolation of alpha-latrotoxin using high-performance liquid chromatography methods].

alpha-Latrotoxin, a main toxic component of the Latrodectus mactans tredecimguttatus venom is a large polypeptide with molecular weight of 130 KDa. A rapid method is suggested for isolating this protein using high-effective liquid chromatography on chromatograph FPLC ("Pharmacia", Sweden). The isolated protein does not differ from the previously described alpha-latrotoxin in the main physicochemical parameters as well as in physiological properties.