High quality chromosome-level genome assembly of Psammosilene tunicoides (Caryophyllaceae), an endangered medicinal plant.

Psammosilene tunicoides, a medicinal plant with significant anti-inflammatory and analgesic properties, is currently classified in China as a Vulnerable species and a Grade II Protected Wild Plant. In this study, a chromosome-level de novo genome assembly of P. tunicoides was performed for the first time, yielding a total genome size of approximately 1,463,250,424 bp, with a contig N50 of 91,918,445 bp and a scaffold N50 of 103,834,185 bp. Approximately 94.40% of the assembled sequences were anchored onto 14 pseudochromosomes. A total of 30,924 genes were predicted, with more than 95% of the genes annotated. Repetitive elements account for 83.40% of the genome. BUSCO analysis indicated high completeness for both the assembly and annotation, with scores of 98.40% and 98.60%, respectively. This high-quality genomic resource establishes a foundation for exploring adaptive evolution and genomic diversity in P. tunicoides and its relatives, while also supporting genetic improvement, resource conservation, and utilization initiatives.