Multiple nitrogenous constituents from Moringa Oleifera leaves and their neuroprotective activity by PC12 cell model in vitro.

To isolate and identify new types of nitrogenous constituents from leaves of Moringa oleifera, as well as to further investigate their potential cytoprotective effects against H2O2-induced cellular injury, thirteen secondary metabolites were purified included one new nitrile glycoside (1), one new pyrrole alkaloid (2), and eleven nitrogenous phytoconstituents (3-13), determined based on chemical and spectroscopic data, such as 1D and 2D NMR, and mass spectra. Among them, the protective effects of compounds 1-13 on adrenal pheochromocytoma (PC12) cells exposed to hydrogen peroxide were evaluated. Compound 2 showed significant neuroprotective activity at concentrations of 1, 5, and 10 μM. Compounds 5 and 9 showed significant neuroprotective activity at concentrations of 5 and 10 μM. Compound 10 displayed significant neuroprotective activity against H2O2-induced cell damage at a concentration of 10 μM. In general, nitrogenous compounds from Moringa Oleifera leaves exhibited a potential cytoprotective effect against H2O2-induced cellular injury.