Anxiogenic activity of centrally administered scorpion (Mesobuthus tamulus) venom in rats.

The anxiogenic action of intracerebroventricularly (i.c.v.) administered Mesobuthus tamulus venom (MTV) was investigated in rats. MTV (1, 3 and 5 g/rat, i.c.v.) induced a dose-related anxiogenic response which was qualitatively comparable to that produced by yohimbine (2 mg/kp, i.p.), an established anxiogenic agent. Both MTV and yohimbine reduced exploratory activity and rears, while increasing immobility and defaecation, in the open-field test. Likewise, both the drugs decreased the number of entries and time spent on the open arms of the elevated plus-maze, reduced social interaction and increased feed-latency in a novel environment. Rat brain levels of tribulin, a postulated endocoid indicator of anxiety, assessed in terms of endogenous monoamine oxidase (MAO) A and B inhibitor activity, were increased by both MTV and yohimbine. The results indicate that the venom has significant anxiogenic activity, which is consonant with some of the clinical symptoms seen after scorpion sting.