Steroidal alkaloids from the rhizomes of Veratrum stenophyllum with anti-inflammatory potential.

4 new isosteroidal alkaloids (1-4) and 21 known analogues (5-25) were isolated from Veratrum stenophyllum rhizomes. At a concentration of 5 μM, compounds 1, 2, 5, and 15 inhibited nitric oxide production and lactate dehydrogenase release in lipopolysaccharide-induced RAW 264.7 macrophages. Notably, compounds 2 and 15 reduced intracellular reactive oxygen species levels and the mRNA expression of pro-inflammatory cytokines.