Intelligent identification of foodborne pathogenic bacteria by self-transfer deep learning and ensemble prediction based on single-cell Raman spectrum.

Foodborne pathogenic infections pose a significant threat to human health. Accurate detection of foodborne diseases is essential in preventing disease transmission. This study proposed an AI model for precisely identifying foodborne pathogenic bacteria based on single-cell Raman spectrum. Self-transfer deep learning and ensemble prediction algorithms had been incorporated into the model framework to improve training efficiency and predictive performance, significantly improving prediction results. Our model can identify simultaneously gram-negative and positive, genus, species of foodborne pathogenic bacteria with an accuracy over 99.99 %, as well as recognized strain with over 99.49 %. At all four classification levels, unprecedented excellent predictive performance had been achieved. This advancement holds practical significance for medical detection and diagnosis of foodborne diseases by reducing false negatives.