Mitochondrial targeted fluorescent nitrite peroxide probe for dynamic monitoring of cellular lung injury.

Herein, a mitochondrial targeted fluorescent nitrite peroxide probe CHP for dynamic monitoring of cellular lung injury was developed. For the practical delivery and selectivity, the structural features including pyridine head and borate recognition group were selected. CHP could respond to ONOO- with the 585 nm fluorescence signal. The detecting system indicated advantages such as wide linear range (0.0-30 μM), high sensitivity (LOD = 0.18 μM), high selectivity and steadiness under different environmental conditions including pH (3.0-10.0), time (48 h) and medium. In living A549 cells, the response of CHP towards ONOO- showed dose-dependent and time-dependent tendencies. The co-localization suggested that CHP could achieve mitochondrial targeting. Moreover, CHP could monitor the variation of endogenous ONOO- level and the cellular lung injury induced by LPS.