Streptomyces haneummycinicus sp. nov., a new antibiotic-producing actinomycete isolated from marine sediment.

An actinomycete strain that produces a new antibiotic haneummycin, designated KM77-8T, was isolated from marine sediment collected from Tokyo Bay near Haneda Airport, Japan. This strain was phylogenetically related to Streptomyces pilosus NBRC 12807T, with the highest similarity (99.65%) based on 16S rRNA gene sequences. In contrast, genome-based comparisons between strain KM77-8T and S. pilosus JCM 4403T (=NBRC 12807T) revealed that average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values were 87.29% and 33.00 %, respectively. Moreover, genome-based comparisons between strain KM77-8T and other known Streptomyces species, including close relatives (more than 98.7% similarities of 16S rRNA gene sequences), showed that their ANI and dDDH values were <90% and <70%, respectively. The DNA G+C content of strain KM77-8T was 71.34%. The whole-cell hydrolysates of strain KM77-8T contained ll-diaminopimelic acid, galactose, glucose, mannose and ribose. The major menaquinones (MKs) of this strain were MK-9(H6) and MK-9(H8), and the major fatty acids (>10%) were anteiso-C15 : 0, iso-C15 : 0, C16 : 0, iso-C16 : 0 and summed feature 3 (C16 : 1  ω7c/C16 : 1  ω6c). Several phenotypic differences were observed between strain KM77-8T and S. pilosus NBRC 12807T. Furthermore, supernatant and methanol extract of not S. pilosus NBRC 12807T but strain KM77-8T displayed antibacterial activity against Enterococcus faecium and contained haneummycin. On the basis of the polyphasic data, we propose strain KM77-8T as a novel species of the genus Streptomyces, named Streptomyces haneummycinicus sp. nov. The type strain is KM77-8T (=NBRC 115995T=DSM 115857T).