A novel pyrrolidine alkaloid and a novel isoflavone with anti-inflammatory activity from the tubers of Arisaema franchetianum.

Solanpyrrole B (1), a new pyrrolidine alkaloid and 5,7,4',5'-tetrahydroxy-6, 3'-dimethoxyisoflavone (2), a new isoflavone, along with 12 known compounds (3-14), were isolated from the tubers of Arisaema franchetianum. The chemical structures of these compounds were identified by using spectroscopic analysis (NMR, UV, IR, HR-ESI-MS). Compounds 1 and 2 (10 μM) exhibited anti-inflammatory activities in inhibiting the NF-κB signaling pathway, reducing the release of NO from RAW264.7 macrophages and BV2 microglia induced by LPS. Compounds 1, 2 and 11 exhibited significant cytotoxic activity against HCT116 cells (IC50 = 9.16 ± 1.59 μM), HGC27 cells (IC50 = 7.98 ± 1.07 μM), U87MG (IC50 = 2.81 ± 0.18 μM), respectively.