Acta parasitologica · 2026
Axenic Cultivation of the First Human-Derived Breviata anathema from a Patient with Fascioliasis in West Bengal, India.
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Sardar Sanjib Kumar, Ghosal Ajanta, Saito-Nakano Yumiko, Suzuki Jun, Ganguly Sandipan, Kobayashi Seiki
Аннотация
PURPOSE: To establish axenic cultivation of a human-derived Breviata anathema (strain T76) isolated from the stool of a patient with fascioliasis and to characterize its biological properties.
METHODS: T76 was maintained in American Type Culture Collection (ATCC) medium 207, supplemented with sulfate-reducing Desulfovibrio desulfuricans and co-cultured with Pseudomonas aeruginosa, at 30 °C. Axenic cultivation was attempted by transferring T76 flagellates to various axenic culture media supplemented with antibiotics for parasitic intestinal protozoan species.
RESULTS: The 18 S rRNA gene sequence of T76 exhibited significant homology with the free-living, bacterivorous B. anathema (ATCC 50338), a member of the rare clade Obazoa. Axenic cultivation of B. anathema (T76) was achieved in YIGADHA-S medium, in which gluconic acid and dihydroxyacetone replaced glucose as energy substrates.
CONCLUSION: B. anathema (T76) was isolated from a patient with fascioliasis, exposed through consumption of aquatic plants from ponds, a known habitat of this organism. Axenic cultivation of T76 was established in YIGADHA-S medium, originally developed for Entamoeba dispar, and cyst-like spherical bodies harboring flagellates were observed and exhibited resistance to 0.1 N HCl.
METHODS: T76 was maintained in American Type Culture Collection (ATCC) medium 207, supplemented with sulfate-reducing Desulfovibrio desulfuricans and co-cultured with Pseudomonas aeruginosa, at 30 °C. Axenic cultivation was attempted by transferring T76 flagellates to various axenic culture media supplemented with antibiotics for parasitic intestinal protozoan species.
RESULTS: The 18 S rRNA gene sequence of T76 exhibited significant homology with the free-living, bacterivorous B. anathema (ATCC 50338), a member of the rare clade Obazoa. Axenic cultivation of B. anathema (T76) was achieved in YIGADHA-S medium, in which gluconic acid and dihydroxyacetone replaced glucose as energy substrates.
CONCLUSION: B. anathema (T76) was isolated from a patient with fascioliasis, exposed through consumption of aquatic plants from ponds, a known habitat of this organism. Axenic cultivation of T76 was established in YIGADHA-S medium, originally developed for Entamoeba dispar, and cyst-like spherical bodies harboring flagellates were observed and exhibited resistance to 0.1 N HCl.