International journal of molecular sciences · 2025
Establishing Bovine Embryonic Stem Cells and Dissecting Their Self-Renewal Mechanisms.
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Li Ningxiao, Yang Zhen, Su Yue, Ma Wei, Zhao Jianglin, Wang Xiangyan, Wan Wenjing, Xie Shengcan, Li Heqiang, Wang Ming, Zhao Yiyu, Han Shiyao, Li Tianle, Xiehe Shuangyi, Guo Jintong, Yue Linxiu, Li Xiaoting, Wang Ahui, Jiang Fenfen, Qing Suzhu, Liu Xinfeng, Liu Jun, Lei Anmin, Tang Young
Аннотация
Bovine pluripotent stem cells (PSCs) hold significant potential for diverse applications in agriculture, reproductive biotechnology, and biomedical research. However, challenges persist in establishing stable bovine PSC lines and understanding the mechanisms underlying their pluripotency maintenance. Here, we derived bovine embryonic stem cells (bESCs) from Holstein cattle embryos. These cells exhibited robust differentiation capacity into three germ layers in vitro and in vivo. Transcriptome analysis revealed distinct molecular profiles compared to primed-state bESCs. Notably, bESC proliferation ceased on methanol-treated feeder cells, in contrast to mouse ESCs (mESCs), which proliferated normally. Pathway analysis identified key signaling events critical for bESC survival and proliferation, highlighting species-specific regulatory mechanisms. Furthermore, the derived bESCs demonstrated chimerism capacity in early bovine embryos, underscoring their functional pluripotency. This work provides a foundation for advancing bovine embryology research and stem cell-based biotechnologies in livestock.