Nephron. Experimental nephrology · 2010
Differential expression of renal proteins in a rodent model of Meckel syndrome.
СтатьяПоддержка NIH: внешняя программаНазвание и аннотация: Перевод в очереди
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Mason Stephen B, Lai Xianyin, Ringham Heather N, Bacallao Robert L, Harris Peter C, Witzmann Frank A, Gattone Vincent H
Аннотация
BACKGROUND: Meckel syndrome (MKS) is a fatal autosomal recessive condition with prominent renal cystic pathology. Renal protein misexpression was evaluated in the Wpk rat model of human MKS3 gene disease to identify biomarkers for the staging of renal cystic progression.
METHODS: Misexpressed proteins were compared between early and late stages of MKS renal cystic disease using proteomic analysis (two-dimensional gel electrophoresis with LC-MS/MS identification) followed by Western blot analysis.
RESULTS: A proteomic analysis identified 76 proteins with statistically different, normalized abundance in at least one group. Subsequently, Western blot was used to confirm differential expression in several of these and polycystic kidney disease (PKD)-associated proteins. Galectin-1 and vimentin were identified as overexpressed proteins, which have been previously found in the jck mouse model of nephronophthisis 9. Ciliopathic PKD proteins, polycystins 1 & 2, and fibrocystin were also differentially expressed in Wpk kidney.
CONCLUSION: In the Wpk rat, misexpressed proteins were identified that were also implicated in other forms of cystic disease. Numerous proteins were either over- or underexpressed in late-stage disease. Differences in protein expression may serve as biomarkers of cystic disease and its progression.
METHODS: Misexpressed proteins were compared between early and late stages of MKS renal cystic disease using proteomic analysis (two-dimensional gel electrophoresis with LC-MS/MS identification) followed by Western blot analysis.
RESULTS: A proteomic analysis identified 76 proteins with statistically different, normalized abundance in at least one group. Subsequently, Western blot was used to confirm differential expression in several of these and polycystic kidney disease (PKD)-associated proteins. Galectin-1 and vimentin were identified as overexpressed proteins, which have been previously found in the jck mouse model of nephronophthisis 9. Ciliopathic PKD proteins, polycystins 1 & 2, and fibrocystin were also differentially expressed in Wpk kidney.
CONCLUSION: In the Wpk rat, misexpressed proteins were identified that were also implicated in other forms of cystic disease. Numerous proteins were either over- or underexpressed in late-stage disease. Differences in protein expression may serve as biomarkers of cystic disease and its progression.
Предметные рубрики MeSH
AnimalsBlotting, WesternCiliary Motility DisordersDisease Models, AnimalElectrophoresis, Gel, Two-DimensionalEncephaloceleFatty Acid Binding Protein 3Fatty Acid-Binding ProteinsFemaleGalectinsHumansKidneyLamininMaleMicePolycystic Kidney DiseasesProteomeProteomicsRatsRats, Mutant StrainsReceptors, Cell SurfaceTRPP Cation ChannelsTandem Mass SpectrometryVimentin