Low cost and renewable H2S-biofilter inoculated with Trichoderma harzianum.

The use of biogas to produce hydrogen is currently gaining more attention. One of the drawbacks for the valorization of biogas is the presence of H2S, a hazardous molecule that can cause damage in the metallic internal structures of industries. In this study, the H2S-removal performance of a fungi-based biofilter was investigated. First, an H2S-resistant fungal species was isolated from an industrial digestate and identified as Trichoderma harzianum. The capacity of this microorganism to metabolize H2S in a mineral medium was confirmed. Then, a bioreactor was constructed and put in place to monitor the elimination of gaseous H2S. A mix of cardboard, perlite, woodchips, and wood pellets was used as filling. Microbial development and the outlet gas composition were monitored during a 60-day experimental process during which H2S was completely removed. 97% of the introduced sulphur was detected in the used filling material (fungal species + packing material) by elemental analysis. 24% of the detected sulphur was identified by ion-exchange chromatography as SO42-. Elemental analysis, gas chromatography, and ion-exchange chromatography were used to determine the bioreactor sulphur balance. Metagenomic analysis underlined that H2S elimination was due to the presence of Trichoderma harzianum with a H2S-specific bacterial consortium.