[Clinical pathology of Dubin-Johnson syndrome].

OBJECTIVE

To investigate the property of pigment granules in the hepatocytes in patients with Dubin-Johnson syndrome.

METHOD: Light microscopy, histochemical, immnohistochemical and electron microscopy techniques were used to study the pigment glanules and the expression of S-100 protein and HMB45 in hepatocytes.

RESULTS

Histological examination revealed normal lobular architecture and the abundont brown pigments which were chiefly seen in the centrilobular zone hepatocytes. The pigment granules were evidenced to have the characterization of both lipofuscin and melanin by histochemical staining and ultrastructural studies and to have the featurts of the melanin by immnohistochemical staining.

CONCLUSION

The results suggest that the pigment graunles are lipfuscin-melanin complex in the hepatocytes in patients with Dubin-Johnson syndrome.