Modification of the acetylcholinesterase (AchE) staining method in Hirschsprung's disease.

Demonstration of the increasing activity of acetylcholinesterase (AChE) in a segment of the colon has proved to be the most accurate diagnostic tool to diagnose Hirschsprung's disease. Two methods of histochemical assessment were tried to establish the most appropriate and effective method for this study within the limitation of available equipment. Lake's method was chosen and was modified as the standard histochemical examination.