Japanese journal of infectious diseases · 2004
Spotted fever group rickettsiae from ticks captured in Sudan.
СтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод в очереди
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Morita Chiharu, El Hussein Abdel Rahim Mohamed, Matsuda Etsurou, Abdel Gabbar Khalil Mohamed Ali, Muramatsu Yasukazu, Abdel Rahman Magdi Badawi, Eleragi Ali Mohamed Hassan, Hassan Shawgi Mohamed, Chitambo Agnes Mumbi, Ueno Hiroshi
Аннотация
Ticks were collected from ruminants in various areas of Sudan in 1998 and 2000. Primer pairs of rickettsial citrate synthase gene (gltA) and a spotted fever group (SFG) rickettsial 190-kDa surface antigen gene (rompA), respectively, were used for identification. Polymerase chain reaction (PCR)-positive products were used for DNA sequencing. The gltA gene was detected in 55% of the ticks examined (57/104). Among the 57 ticks studied, 19 were positive for the rompA gene. Thus, 18% of the ticks examined were found to be infected with SFG rickettsiae. The nucleotide sequences of the amplified rompA gene fragment of Hyalomma spp. and Amblyomma spp. were similar to those of Rickettsia aeschlimannii and Rickettsia africae, respectively. In this study, we succeeded in detecting the SFG rickettsiae gene in ticks, and established that there were at least two species of SFG rickettsiae in field ticks in Sudan.