Eutymeroterpenoids A-G with cytotoxic and anti-inflammatory activity from the fungus Eutypella scoparia GZU-4-19Y.

Eutymeroterpenoids A-G (1-7), seven new eremophilane-type sesquiterpenoids, together with one known compound cryptosphaerolide (8), were isolated from marine-derived Eutypella scoparia GZU-4-19Y. Their structures were established by analysis of their NMR data and electronic circular dichroism calculations. All of them featured the unique 11‑carbon alkyl chain were rarely found in natural sesquiterpenoids. The isolates were evaluated for their anti-cancer and anti-inflammatory activities in vitro. Compounds 4, 5, 7 and 8 exhibited moderate cytotoxic activities against two human cancer cell lines (HT29 and HepG2). In addition, compounds 5 and 8 displayed good inhibitory activities against lipopolysaccharide-induced NO production in RAW264.7 cells with IC50 values of 26.9 and 13.7 μM, respectively, comparable to that of the positive control indomethacin (IC50 value = 24.2 μM). Western blot analysis further indicated that compounds 5 and 8 decreased the level of pro-inflammatory enzymes iNOS and COX-2.