Molecular-genetic characteristics of hmgb1 mrna expression in blood of women with endometriosis associated with infertility.

OBJECTIVE

Aim: To examine HMGB1 expression in the blood of women with endometriosis-associated infertility and to establish its role in disease progression.

PATIENTS AND

METHODS

Materials and

Methods

We analyzed HMGB1 gene expression levels in two groups: 20 women with endometriosis-associated infertility (main group) and 10 healthy women (control group). The study was conducted at Bukovinian State Medical University and the Centre of Reproductive Medicine. Primary infertility was significantly higher in the main group. We used real-time reverse transcription polymerase chain reaction (RT-PCR) to determine HMGB1 mRNA levels in mononuclear cells isolated from whole blood. Statistical significance was determined using the Student's t-test, with p < 0.05 considered significant.

RESULTS

Results

Results indicated a significant increase in HMGB1 mRNA expression in the main group compared to the control group (p < 0.001). The relative normalized expression ranged from 1.8924 to 33.426 (median = 8.01). Expression levels were categorized into three groups: borderline with the control, moderate increase, and significant increase. Only one sample (5%) had values equal to the control. Fifteen percent of samples had values slightly above control (1.89-3.45), 30% had moderate increases (3.45-8.01), and 50% had significant increases (>8.01). In 95% of the main group, HMGB1 mRNA expression was elevated, predominantly at high values (p < 0.001).

CONCLUSION

Conclusions

Women with endometriosis-associated infertility show significantly increased HMGB1 mRNA expression, particularly in moderate and severe cases compared to mild cases, indicating HMGB1's role in disease progression (p < 0.001).