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DOI 10.1016/j.scitotenv.2021.152345

Медицинская библиотека · The Science of the total environment · 2021

Toxicity of spike fragments SARS-CoV-2 S protein for zebrafish: A tool to study its hazardous for human health?

Ventura Fernandes Bianca H, Feitosa Natália Martins, Barbosa Ana Paula, Bomfim Camila Gasque, Garnique Anali M B, Rosa Ivana F, Rodrigues Maira S, Doretto Lucas B, Costa Daniel F, Camargo-Dos-Santos Bruno, Franco Gabrielli A, Neto João Favero, Lunardi Juliana Sartori, Bellot Marina Sanson, Alves Nina Pacheco Capelini, Costa Camila C, Aracati Mayumi F, Rodrigues Letícia F, Costa Camila C, Cirilo Rafaela Hemily, Colagrande Raul Marcelino, Gomes Francisco I F, Nakajima Rafael T, Belo Marco A A, Giaquinto Percília Cardoso, de Oliveira Susana Luporini, Eto Silas Fernandes, Fernandes Dayanne Carla, Manrique Wilson G, Conde Gabriel, Rosales Roberta R C, Todeschini Iris, Rivero Ilo, Llontop Edgar, Sgro Germán G, Oka Gabriel Umaji, Bueno Natalia Fernanda, Ferraris Fausto K, de Magalhães Mariana T Q, Medeiros Renata J, Mendonça-Gomes Juliana M, Junqueira Mara Souza, Conceição Kátia, Pontes Leticia Gomes de, Condino-Neto Antonio, Perez Andrea C, Barcellos Leonardo J G, Júnior José Dias Correa, Dorlass Erick Gustavo, Camara Niels O S, Durigon Edison Luiz, Cunha Fernando Q, Nóbrega Rafael H, Machado-Santelli Glaucia M, Farah Chuck S, Veras Flavio P, Galindo-Villegas Jorge, Costa-Lotufo Letícia V, Cunha Thiago M, Chammas Roger, Carvalho Luciani R, Guzzo Cristiane R, Malafaia Guilherme, Charlie-Silva Ives

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Аннотация

Despite the significant increase in the generation of SARS-CoV-2 contaminated domestic and hospital wastewater, little is known about the ecotoxicological effects of the virus or its structural components in freshwater vertebrates. In this context, this study evaluated the deleterious effects caused by SARS-CoV-2 Spike protein on the health of Danio rerio, zebrafish. We demonstrated, for the first time, that zebrafish injected with fragment 16 to 165 (rSpike), which corresponds to the N-terminal portion of the protein, presented mortalities and adverse effects on liver, kidney, ovary and brain tissues. The conserved genetic homology between zebrafish and humans might be one of the reasons for the intense toxic effects followed inflammatory reaction from the immune system of zebrafish to rSpike which provoked damage to organs in a similar pattern as happen in severe cases of COVID-19 in humans, and, resulted in 78,6% of survival rate in female adults during the first seven days. The application of spike protein in zebrafish was highly toxic that is suitable for future studies to gather valuable information about ecotoxicological impacts, as well as vaccine responses and therapeutic approaches in human medicine. Therefore, besides representing an important tool to assess the harmful effects of SARS-CoV-2 in the aquatic environment, we present the zebrafish as an animal model for translational COVID-19 research.

Для цитирования: Ventura Fernandes Bianca H, Feitosa Natália Martins, Barbosa Ana Paula, Bomfim Camila Gasque, Garnique Anali M B, Rosa Ivana F, Rodrigues Maira S, Doretto Lucas B, Costa Daniel F, Camargo-Dos-Santos Bruno, Franco Gabrielli A, Neto João Favero, Lunardi Juliana Sartori, Bellot Marina Sanson, Alves Nina Pacheco Capelini, Costa Camila C, Aracati Mayumi F, Rodrigues Letícia F, Costa Camila C, Cirilo Rafaela Hemily, Colagrande Raul Marcelino, Gomes Francisco I F, Nakajima Rafael T, Belo Marco A A, Giaquinto Percília Cardoso, de Oliveira Susana Luporini, Eto Silas Fernandes, Fernandes Dayanne Carla, Manrique Wilson G, Conde Gabriel, Rosales Roberta R C, Todeschini Iris, Rivero Ilo, Llontop Edgar, Sgro Germán G, Oka Gabriel Umaji, Bueno Natalia Fernanda, Ferraris Fausto K, de Magalhães Mariana T Q, Medeiros Renata J, Mendonça-Gomes Juliana M, Junqueira Mara Souza, Conceição Kátia, Pontes Leticia Gomes de, Condino-Neto Antonio, Perez Andrea C, Barcellos Leonardo J G, Júnior José Dias Correa, Dorlass Erick Gustavo, Camara Niels O S, Durigon Edison Luiz, Cunha Fernando Q, Nóbrega Rafael H, Machado-Santelli Glaucia M, Farah Chuck S, Veras Flavio P, Galindo-Villegas Jorge, Costa-Lotufo Letícia V, Cunha Thiago M, Chammas Roger, Carvalho Luciani R, Guzzo Cristiane R, Malafaia Guilherme, Charlie-Silva Ives. Toxicity of spike fragments SARS-CoV-2 S protein for zebrafish: A tool to study its hazardous for human health?. The Science of the total environment. 2021. DOI: 10.1016/j.scitotenv.2021.152345.

Предметные рубрики MeSH

AnimalsCOVID-19FemaleHumansSARS-CoV-2Spike Glycoprotein, CoronavirusZebrafish
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