Displacement chromatography on cyclodextrin-silicas. III. Enantiomer separations.

The feasibility of preparative enantiomer separations by displacement chromatography on analytical-scale beta-cyclodextrin-silica columns, operated in the reversed-phase mode, was demonstrated using the enantiomers of mephobarbital, hexobarbital, dansylleucine, dansylvaline and dansylphenylalanine as model solutes. The method development scheme (which relies on the determination of the elution-mode retention behavior and the adsorption isotherms of the solutes and the candidate displacers) described in Parts I and II can be used to select the appropriate displacers and the conditions leading to a successful displacement chromatographic separation. The importance of the displacer (both type and concentration) to the success of the displacement chromatographic separation is demonstrated.