Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology · 2016
[Decreased expression levels of LAG-3 and CD49b on CD14+ cells in peripheral blood of patients with recurrent spontaneous abortion].
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Zeng Hanyu, Liu Yuan, Guo Wenwei, Wang Xiaohong, Chen Lihua
Аннотация
OBJECTIVE: To investigate the expression levels of adhesion molecule CD49b and negative regulation molecule LAG-3 (CD223) on peripheral blood CD14(+) cells in patients with recurrent spontaneous abortion (RSA).
METHODS: Peripheral blood samples were collected from 7 normal female individuals and 12 patients with RSA, and then peripheral blood mononuclear cells (PBMCs) and plasma were separated from the peripheral blood via centrifugation. The expression levels of CD49b and LAG-3 on CD14(+) cells in PBMCs were detected by flow cytometry and the levels of interleukin 10 (IL-10) and transforming growth factor β (TGF-β) in plasma were detected by ELISA.
RESULTS: In the RSA patient group, there was no significant difference in the percentage of monocytes compared with that of the normal female group. The numbers of CD14(+) CD49b(+), CD14(+) LAG-3(+) and CD14(+) CD49b(+) LAG-3(+) cells in the RSA patient group were lower than those of the normal female group. The plasma level of TGF-β in the RSA patient group was lower than that of the normal female group. However, there was no significant difference in the plasma level of IL-10 between the two groups.
CONCLUSION: In RSA patients, the expression levels of CD49b and LAG-3 on CD14(+) monocytes and the plasma level of TGF-β decreased obviously compared with that of the normal females.
METHODS: Peripheral blood samples were collected from 7 normal female individuals and 12 patients with RSA, and then peripheral blood mononuclear cells (PBMCs) and plasma were separated from the peripheral blood via centrifugation. The expression levels of CD49b and LAG-3 on CD14(+) cells in PBMCs were detected by flow cytometry and the levels of interleukin 10 (IL-10) and transforming growth factor β (TGF-β) in plasma were detected by ELISA.
RESULTS: In the RSA patient group, there was no significant difference in the percentage of monocytes compared with that of the normal female group. The numbers of CD14(+) CD49b(+), CD14(+) LAG-3(+) and CD14(+) CD49b(+) LAG-3(+) cells in the RSA patient group were lower than those of the normal female group. The plasma level of TGF-β in the RSA patient group was lower than that of the normal female group. However, there was no significant difference in the plasma level of IL-10 between the two groups.
CONCLUSION: In RSA patients, the expression levels of CD49b and LAG-3 on CD14(+) monocytes and the plasma level of TGF-β decreased obviously compared with that of the normal females.