Determination of carboquone in plasma and ascites by high performance liquid chromatography after intravenous and intraperitoneal administration in man.

A new method for the determination of carboquone (CQ) in human plasma and ascites by high performance liquid chromatography has been developed. The method is sensitive enough to determine levels as low as 1.0 ng per 1.0 ml of plasma or ascites. The average recovery of CQ from these samples was 89.3 +/- 0.9% (n = 5, mean +/- SD). The method consists of 2 steps, 1) extracting CQ from human body fluids with chloroform and 2) quantization of CQ by chromatography using a mu-Bondapak C18 column, monitored at 340 nm, 2,5-Diethyleneimino-3,6-dimethylbenzoquinone was used as the internal standard. Using this analytical method, the concentration-time profiles of CQ in human body fluids after intravenous and intraperitoneal administrations were obtained. It was recognized that intraperitoneal administration of CQ was advantagenous for the treatment of peritonitis carcinomatosa since an effective concentration could be maintained in ascites for about 6 hr.