Fertility and sterility · 2004
Successful fertilization and pregnancy after intracytoplasmic sperm injection and oocyte activation with calcium ionophore in a normozoospermic patient with extremely low fertilization rates in intracytoplasmic sperm injection cycles.
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Chi Hee-Jun, Koo Jung-Jin, Song Sang-Jin, Lee Ji-Youn, Chang Sang-Sik
Аннотация
OBJECTIVE: To investigate the effect of calcium ionophore on the fertilization rate of a patient with normozoospermia who nonetheless exhibited a low fertilization rate in intracytoplasmic sperm injection (ICSI).
DESIGN: Case report.
SETTING: In vitro fertilization center.
PATIENT(S): A male patient whose sperm, though diagnosed as normal by semen analysis, exhibited a severely low fertilization rate in ICSI cycles.
INTERVENTION(S): Oocytes were activated by calcium ionophore after ICSI.
MAIN OUTCOME MEASURE(S): Fertilization rate after oocyte activation; ultrastructure and protein expression of the patient's sperm.
RESULT(S): The fertilization rate of oocytes activated with calcium ionophore (12 of 15, 80.0%) was higher than that of the nonactivated oocytes (4 of 16, 25.0%). Four embryos derived from the activated oocytes were transferred, resulting in a twin pregnancy. Further investigation revealed abnormalities in the patient's sperm: many nuclear vacuoles were observed and the expression of some proteins was absent.
CONCLUSION(S): Oocyte activation with calcium ionophore was effective at increasing the fertilization rate of dysfunctional sperm characterized by ultrastructural and protein expression anomalies.
DESIGN: Case report.
SETTING: In vitro fertilization center.
PATIENT(S): A male patient whose sperm, though diagnosed as normal by semen analysis, exhibited a severely low fertilization rate in ICSI cycles.
INTERVENTION(S): Oocytes were activated by calcium ionophore after ICSI.
MAIN OUTCOME MEASURE(S): Fertilization rate after oocyte activation; ultrastructure and protein expression of the patient's sperm.
RESULT(S): The fertilization rate of oocytes activated with calcium ionophore (12 of 15, 80.0%) was higher than that of the nonactivated oocytes (4 of 16, 25.0%). Four embryos derived from the activated oocytes were transferred, resulting in a twin pregnancy. Further investigation revealed abnormalities in the patient's sperm: many nuclear vacuoles were observed and the expression of some proteins was absent.
CONCLUSION(S): Oocyte activation with calcium ionophore was effective at increasing the fertilization rate of dysfunctional sperm characterized by ultrastructural and protein expression anomalies.