Chemical composition of the endogenous fungus Fusarium sp. L-3 derived from Chinese Qinan agarwood.

One undescribed compound named fusagarone A (1) and ten secondary metabolites (2-11) were isolated from the endophytic fungus Fusarium sp. L-3 derived from Chinese agarwood 'Qi-Nan'. The structure of 1 was elucidated by NMR spectroscopy as well as HRESIMS data. All compounds were measured for anti-inflammatory, cytotoxicity activity and α-glucosidase inhibitory activities. Among them, compound 11 potently suppressed nitric oxide (NO) production on lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages with IC50 value of 4.78 μM. Moreover, compound 11 exhibited potent inhibitory effect on α-glucosidase with IC50 value of 42.88 μM, which was further clarified by means of the enzyme kinetic analysis and molecular docking simulations.