[DNA damage induced long non-coding RNA (DINO) down-regulates the expression of MMP2 and inhibits the invasion and migration of human chorionic trophoblast cells].

Objective To investigate the effect of DNA damage induced long non-coding RNA (DINO) on the invasion and migration of human chorionic trophoblast cells via its regulating the expression of migration-related factor matrix metalloproteinase 2 (MMP2). Methods The placental tissues of 20 patients with preeclampsia (PE) and 20 normal pregnant women (control group) were collected. The changes of placenta structure were observed by Masson staining. The expression of MMP2 in placental trophoblast cells was detected by immunofluorescence histochemical staining. The expression of DINO was detected by real-time fluorescence quantitative PCR. After DINO small interference fragments (si-DINO) were transfected into HTR-8/Svneo human chorionic trophoblast cells, the expressions of DINO and MMP2 mRNA were detected by real-time fluorescent quantitative PCR, and the expression of MMP2 protein was detected by Western blot. The invasion and migration abilities of cells were detected by TranswellTM invasion assay and cell scratch assay. Results Compared with the normal pregnant women, the PE patients' blood pressure and proteinuria levels were significantly increased; fibrinoid necrosis and chorionic villi reduction were observed in the placental tissue; immunofluorescence showed that the expression of MMP2 was significantly decreased and the expression of DINO was increased in placental trophoblast cells; after transfection with si-DINO, the expression of DINO was decreased, while the expressions of MMP2 mRNA and protein were increased, and the invasion and migration abilities of HTR-8/Svneo cells increased significantly. Conclusion DINO down-regulates the expression of MMP2 and inhibits the invasion and migration of placental trophoblast cells.