Zhurnal mikrobiologii, epidemiologii i immunobiologii · 2010
[Improvement of method of Coxiella burnetii detection in biological material based on groEL gene amplification].
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Freĭlikhman O A, Panferova Iu A, Tokarevich N K
Аннотация
AIM: Improvement of PCR for detection of Coxiella burnetii in field material by development of set of primers for amplification of groEL gene fragment.
MATERIALS AND METHODS: C. burnetii strains, samples from organs of wild rodents and laboratory animals, blood of healthy donors and laboratory animals. Methods of DNA isolation, PCR, and electrophoresis in agarose gel were used.
RESULTS: Nucleotide sequences of primers amplifying groEL gene fragment, which could increase specificity of PCR during work with field material were proposed.
CONCLUSION: Obtained data open perspective for using this method for detection of C. burnetii in environment.
MATERIALS AND METHODS: C. burnetii strains, samples from organs of wild rodents and laboratory animals, blood of healthy donors and laboratory animals. Methods of DNA isolation, PCR, and electrophoresis in agarose gel were used.
RESULTS: Nucleotide sequences of primers amplifying groEL gene fragment, which could increase specificity of PCR during work with field material were proposed.
CONCLUSION: Obtained data open perspective for using this method for detection of C. burnetii in environment.