PROSPECTS FOR THE DEVELOPMENT OF TECHNOLOGY OF ADHESIVE FILMS WITH ASIAN MINT (MENTHA ASIATICA BORISS.) EXTRACT.

UNLABELLED: The results of the development, standardization and biological evaluation of an innovative two-layer mucoadhesive dental film containing Mentha asiatica Boriss. extract and lidocaine hydrochloride for the treatment of inflammatory diseases of the oral cavity are presented.

MATERIALS AND

METHODS

Ethanol extract of Mentha asiatica Boriss. was obtained by Soxhlet extraction followed by freeze-drying. The chemical composition was determined by GC-MS, which revealed 1,8-cineole oxide and piperitenone. The analytical method demonstrated high linearity (R²>0.995), sensitivity (LOD=0.01 μg/ml; LOQ=0.05 μg/ml) and accuracy (RSD<3%).

RESULTS

The optimized film (10% collagen, 10% gelatin, 2% glycerin) showed high elasticity, strength and adhesion. Physicochemical characteristics included: pH 6.5±0.2, swelling time 3-5 min, deviation from homogeneity <7% and complete dissolution in 10-16 min. The in vitro release profile of lidocaine hydrochloride demonstrated an initial rapid release (36.0% within 1 hour), followed by a sustained release reaching 95.0% at 24 hours, confirming the film's ability to provide prolonged local analgesia. Microbiological purity complied with the standards of the Republic of Kazakhstan and the EAEU. Sterilization with ethylene oxide ensured a shelf life of more than 2 years. At a concentration of 31.25 μg/ml, the extract reduced NO production by RAW264.7 macrophages by 31.8%. SAEE exhibited selective cytotoxicity towards cancer cells while maintaining the safety of HUVEC. Compared to analogues (RU2739260C1, RU2734249C2), the resulting film was distinguished by better solubility, increased adhesion, and a unique composition.

CONCLUSION

The developed two-layer mucoadhesive film combines antimicrobial, anti-inflammatory and analgesic effects, has improved physicochemical and biological properties, and can be considered a promising form for industrial production and clinical trials.