Current eye research · 2004
Involvement of CD8+ T-cells in exacerbation of corneal scarring in mice.
СтатьяПоддержка исследования: вне правительства СШАПоддержка службы здравоохранения СШАНазвание и аннотация: Перевод в очереди
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Osorio Yanira, Cai Steve, Hofman Florence M, Brown Donald J, Ghiasi Homayon
Аннотация
PURPOSE: To determine the specific immune responses involved in the exacerbation of corneal scarring induced by HSV-1 in gK vaccinated mice.
MATERIALS AND METHODS: BALB/c mice were vaccinated with HSV-1 glycoprotein K (gK) and ocularly challenged with HSV-1. Infiltration into the cornea of T cells and macrophages was monitored by immunocytochemistry, and the effect of depletion of CD4+ T-cells, CD8+ T-cells, or macrophages on corneal scarring was determined.
RESULTS: Following ocular challenge, CD4+ and CD8+ T-cells and macrophages were more abundant in the corneas of gK-vaccinated mice than in the corneas of mock vaccinated mice. Depletion of CD8+ T-cells, but not of CD4+ T-cells or macrophages, reduced the severity of corneal scarring in gK-vaccinated mice.
CONCLUSIONS: We have shown that gK vaccination causes an overall increase in T cells and macrophages in the cornea after ocular HSV-1 challenge. The immunopathology induced by gK vaccination appears to be related to CD8+ T-cell activity, as depletion of these cells, but not other immune cells, reduced corneal scarring.
MATERIALS AND METHODS: BALB/c mice were vaccinated with HSV-1 glycoprotein K (gK) and ocularly challenged with HSV-1. Infiltration into the cornea of T cells and macrophages was monitored by immunocytochemistry, and the effect of depletion of CD4+ T-cells, CD8+ T-cells, or macrophages on corneal scarring was determined.
RESULTS: Following ocular challenge, CD4+ and CD8+ T-cells and macrophages were more abundant in the corneas of gK-vaccinated mice than in the corneas of mock vaccinated mice. Depletion of CD8+ T-cells, but not of CD4+ T-cells or macrophages, reduced the severity of corneal scarring in gK-vaccinated mice.
CONCLUSIONS: We have shown that gK vaccination causes an overall increase in T cells and macrophages in the cornea after ocular HSV-1 challenge. The immunopathology induced by gK vaccination appears to be related to CD8+ T-cell activity, as depletion of these cells, but not other immune cells, reduced corneal scarring.
Предметные рубрики MeSH
AnimalsAntibodies, MonoclonalAntibody FormationCD4 AntigensCD4-Positive T-LymphocytesCD8 AntigensCD8-Positive T-LymphocytesCicatrixClodronic AcidCorneaCorneal DiseasesEyeFemaleHerpes Simplex Virus VaccinesHerpesvirus 1, HumanImmunity, CellularKeratitis, HerpeticMacrophagesMiceMice, Inbred BALB CVaccinationViral Proteins