[Screening for proteins interacting with novel gene AngRem104 in the adult human kidney cDNA library by yeast two hybrid system].

OBJECTIVE

To screen for proteins interacting with novel gene AngRem104.

METHODS

The yeast strain AH109 was transformed with AngRem104-pGBKT7/c-myc, and yeast-mating was utilized to screen for interacting proteins with AngRem104 in pretransformed human kidney cDNA library.

RESULTS

AngRem104 protein was expressed and there was no self-activation and toxicity in AH109. Seven proteins that interacted with AngRem104, including eukaryotic translation elongation factor 1 alpha 1, glucocorticoid receptor AF-1 specific elongation factor, beta 2-microglobulin, BBS2 and four of cell metabolism related proteins, were identified.

CONCLUSION

The novel gene AngRem104 may interact with the proteins, which may interfere with cell metabolism, disturb the balance between transcription factors, or may be involved in some immunity related and hereditary diseases. This investigation provides functional clues for further exploration of the novel gene, AngRem104.