Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese journal of medical genetics · 2003
[Molecular cloning of TSARG3 gene related to apoptosis in human spermatogenic cells].
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Liu Gang, Lu Guangxiu, Fu Junjiang, Liu Shangfeng, Xing Xiaowei, Li Luyun
Аннотация
OBJECTIVE: To clone a novel gene which is related to human testis spermatogenesis apoptosis.
METHODS: To rapidly attain human novel gene full-length cDNA sequence from a human testis cDNA library,the gene-specific primers and the vector-specific primers were designed for nested polymerase chain reaction. Sequencing was performed and the result was analysed.
RESULTS: The present authors discovered the TSARG3 gene(GenBank accession number AF419291) from a human testis cDNA library, using a cDNA fragment (GenBank accession number BE644537) as an electronic probe, which was significantly changed in cryptorchidism and represented a novel gene. Furthermore, a mouse homologue of this gene was identified (GenBank accession number AF419292) by using the same method.
CONCLUSION: A novel gene named TSARG3 was cloned. It is considered that the function of the new gene is related to human testis spermatogenesis apoptosis.
METHODS: To rapidly attain human novel gene full-length cDNA sequence from a human testis cDNA library,the gene-specific primers and the vector-specific primers were designed for nested polymerase chain reaction. Sequencing was performed and the result was analysed.
RESULTS: The present authors discovered the TSARG3 gene(GenBank accession number AF419291) from a human testis cDNA library, using a cDNA fragment (GenBank accession number BE644537) as an electronic probe, which was significantly changed in cryptorchidism and represented a novel gene. Furthermore, a mouse homologue of this gene was identified (GenBank accession number AF419292) by using the same method.
CONCLUSION: A novel gene named TSARG3 was cloned. It is considered that the function of the new gene is related to human testis spermatogenesis apoptosis.
Предметные рубрики MeSH
Amino Acid SequenceAnimalsApoptosisApoptosis Regulatory ProteinsBase SequenceCloning, MolecularDNA, ComplementaryFemaleGene ExpressionHeat-Shock ProteinsHumansMaleMiceMolecular ChaperonesMolecular Sequence DataProteinsSequence AlignmentSequence Analysis, DNASequence Homology, Amino AcidSpermatocytes