Stem cell reports · 2022
Transcriptional profiling of β-2M-SPα-6+THY1+ spermatogonial stem cells in human spermatogenesis.
СтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод в очереди
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Givelet Maelle, Firlej Virginie, Lassalle Bruno, Gille Anne Sophie, Lapoujade Clementine, Holtzman Isabelle, Jarysta Amandine, Haghighirad Farahd, Dumont Florent, Jacques Sébastien, Letourneur Franck, Pflumio Françoise, Allemand Isabelle, Patrat Catherine, Thiounn Nicolas, Wolf Jean Philippe, Riou Lydia, Barraud-Lange Virginie, Fouchet Pierre
Аннотация
Male infertility is responsible for approximately half of all cases of reproductive issues. Spermatogenesis originates in a small pool of spermatogonial stem cells (SSCs), which are of interest for therapy of infertility but remain not well defined in humans. Using multiparametric analysis of the side population (SP) phenotype and the α-6 integrin, THY1, and β-2 microglobulin cell markers, we identified a population of human primitive undifferentiated spermatogonia with the phenotype β-2 microglobulin (β-2M)-SPα-6+THY1+, which is highly enriched in stem cells. By analyzing the expression signatures of this SSC-enriched population along with other germinal progenitors, we established an exhaustive transcriptome of human spermatogenesis. Transcriptome profiling of the human β-2M-SPα-6+THY1+ population and comparison with the profile of mouse undifferentiated spermatogonia provide insights into the molecular networks and key transcriptional regulators regulating human SSCs, including the basic-helix-loop-helix (bHLH) transcriptional repressor HES1, which we show to be implicated in maintenance of SSCs in vitro.