MedElevator
Войти в исследования PloS one · 2025
Stability, accuracy, and clinical performance of enzymatic total CO₂ measurement: Evaluation of the Snibe and Roche assays.
СтатьяНазвание и аннотация: Перевод в очереди
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Wei Shuai, Fang Zhonggang, Shi Min, Zhang Qunxian, Huang Qiang, Chen Gao, Wei Zhenlei, Sun Chen, Wei Haolin, Li Tinghua, Lan Chen
Аннотация
OBJECTIVES: To evaluate the stability, accuracy, and clinical performance of the Snibe CO₂ assay compared with the Roche CO₂ reagent and to investigate factors affecting total CO₂ measurements in different clinical conditions.
METHODS: Total CO₂ was measured in patient samples using enzymatic assays. Stability was assessed over time, and CO₂ loss was examined in different blood collection tubes. Method comparison was conducted between the Snibe and the Roche CO₂ assays in various diseases.
RESULTS: The Snibe CO₂ assay showed excellent linearity (r = 0.9997) and maintained stability for 42 days without recalibration, with control material deviations within ±5% of the target. CO₂ loss was observed in uncapped non-anticoagulant tubes (24.0% decline over 48 hours), but was less in EP tubes (9.4% decline). Significant differences of median CO₂ measurements were observed in only the renal group (Snibe: 13.35 mmol/L, Roche: 12.80 mmol/L) and the cardiovascular disease group (Snibe: 25.90 mmol/L, Roche: 26.40 mmol/L) versus the healthy group (Snibe: 22.70 mmol/L, Roche: 22.90 mmol/L) for both the Snibe and Roche assays.
CONCLUSIONS: The Snibe CO₂ assay demonstrated superior stability and comparable accuracy compared with the Roche reagent. Preanalytical CO₂ loss remains a critical issue, emphasizing the need for standardized sample handling. Given the correlation between total CO₂ and bicarbonate levels, accurate measurement is critical for diagnosing metabolic disorders. Laboratories should establish protocols to minimize errors and ensure reliable acid-base assessment in clinical practice.
METHODS: Total CO₂ was measured in patient samples using enzymatic assays. Stability was assessed over time, and CO₂ loss was examined in different blood collection tubes. Method comparison was conducted between the Snibe and the Roche CO₂ assays in various diseases.
RESULTS: The Snibe CO₂ assay showed excellent linearity (r = 0.9997) and maintained stability for 42 days without recalibration, with control material deviations within ±5% of the target. CO₂ loss was observed in uncapped non-anticoagulant tubes (24.0% decline over 48 hours), but was less in EP tubes (9.4% decline). Significant differences of median CO₂ measurements were observed in only the renal group (Snibe: 13.35 mmol/L, Roche: 12.80 mmol/L) and the cardiovascular disease group (Snibe: 25.90 mmol/L, Roche: 26.40 mmol/L) versus the healthy group (Snibe: 22.70 mmol/L, Roche: 22.90 mmol/L) for both the Snibe and Roche assays.
CONCLUSIONS: The Snibe CO₂ assay demonstrated superior stability and comparable accuracy compared with the Roche reagent. Preanalytical CO₂ loss remains a critical issue, emphasizing the need for standardized sample handling. Given the correlation between total CO₂ and bicarbonate levels, accurate measurement is critical for diagnosing metabolic disorders. Laboratories should establish protocols to minimize errors and ensure reliable acid-base assessment in clinical practice.