Applied microbiology · 1972
Preparation of purified suspensions of Coxiella burneti by Genetron extraction followed by continuous-flow ultracentrifugation.
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Dubois D R, Cutchins E C, Berman S, Lowenthal J P, Timchak R L
Аннотация
A method for the preparation of purified suspensions of Coxiella burneti by Genetron extraction followed by continuous-flow density gradient ultracentrifugation is described. Both phases of the Henzerling strain of C. burneti were found in a zone between 53 and 65% (w/w) sucrose. Based on chemical assays, the Genetron zonal rickettsial suspensions were found to be as pure as the rickettsial suspensions which were prepared by the ether extraction method currently in use for producing Q fever vaccines for human use.
Предметные рубрики MeSH
AnimalsAntibody FormationAntigens, BacterialBacterial ProteinsBacterial VaccinesBacteriological TechniquesCentrifugation, ZonalChick EmbryoChlorineComplement Fixation TestsCoxiellaEthyl EthersExtraembryonic MembranesFluorineGuinea PigsHydrocarbons, HalogenatedImmunochemistryInjections, IntraperitonealLipidsNitrogenQ FeverUltracentrifugation