[Isolation and HPLC analysis of ginkgolic acid].

OBJECTIVE

To isolate ginkgolic acids (GA) from exopleura of Ginkgo biloba and to analyse GA.

METHODS

GA were ultrasonic-extracted from exopleura of Ginkgo biloba with petroleum ether, and purified by silica gel column chromatography. The content of GA in sample was determined by HPLC with methanol-3% acetic acid (90:10) as mobile phase at a flow rate of 1 ml/min. UV detection wavelength was set at 310 nm. A HiQ sil C18 column was used at 40 degrees C in analysis.

RESULTS

A good linearity was obtained in the range of 1.144-5.720 micrograms (r = 0.9978) for GA. The average recovery of GA was 97.50% with RSD of 1.70%.

CONCLUSION

The method may be employed to prepare GA from exopleura of Ginkgo biloba. The analysis method for GA is accurate and reliable, and can be used for determination of GA in sample.